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Oxford Nanopore native barcode expansion kit (exp-nbd103)
Native Barcode Expansion Kit (Exp Nbd103), supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/native+barcoding+expansion+kit+exp+nbd103/rapid+barcoding+kit+sqk+rbk004/pmc11385720-30-39-49
Average 90 stars, based on 1 article reviews
native barcode expansion kit (exp-nbd103) - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Sequencing:

Article Title: Complete Genome Sequence of a Canadian Klebsiella michiganensis Strain, Obtained Using Oxford Nanopore Technologies Sequencing
Article Snippet: Genomic DNA was extracted and purified from overnight cultures grown in TSB at 37°C using the Nanobind CBB Big DNA kit (cells, bacteria, blood) followed by the Short Read Eliminator XS kit (Circulomics, Inc., Baltimore, MD, USA) and was quantified using a Qubit v3.0 fluorometer (Life Technologies, Thermo Fisher Scientific, Inc., Ottawa, Canada). .. Libraries for MinION sequencing were prepared without shearing using the 1D ligation sequencing kit (SQK-LSK108), and DNA was barcoded with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore Technologies, Oxford, UK). .. The final library was run on a FLO-MIN106 (R9.4.1) flow cell for 48 h on the MinION platform (Oxford Nanopore Technologies).

Article Title: Noninvasive prenatal testing for β-thalassemia by targeted nanopore sequencing combined with relative haplotype dosage (RHDO): a feasibility study
Article Snippet: The size distribution of the libraries was analyzed using an Agilent 2100 bioanalyzer and library concentration was quantified by Qubit (Life Technologies). .. Then the sequencing libraries were prepared employing the Oxford ligation sequencing kit SQK-LSK108 (Oxford Nanopore Technologies) and pooled in equal concentration using native barcoding expansion kit EXP-NBD103 (Oxford Nanopore Technologies) according to recommended procedure. .. The library was subjected to 48 h reads generated on the MK1B MinION platform using the R9.4 (FLO-MIN106) flow cell (Oxford Nanopore Technologies).

Article Title: Complete Genome Sequence of a Canadian Klebsiella michiganensis Strain, Obtained Using Oxford Nanopore Technologies Sequencing
Article Snippet: Genomic DNA was extracted and purified from overnight cultures grown in TSB at 37°C using the Nanobind CBB Big DNA kit (cells, bacteria, blood) followed by the Short Read Eliminator XS kit (Circulomics, Inc., Baltimore, MD, USA) and was quantified using a Qubit v3.0 fluorometer (Life Technologies, Thermo Fisher Scientific, Inc., Ottawa, Canada). .. Libraries for MinION sequencing were prepared without shearing using the 1D ligation sequencing kit (SQK-LSK108), and DNA was barcoded with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore Technologies, Oxford, UK). .. The final library was run on a FLO-MIN106 (R9.4.1) flow cell for 48 h on the MinION platform (Oxford Nanopore Technologies).

Article Title: Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same sequence types reveals diversity in prophages and pathogenicity islands
Article Snippet: Whole-genome sequencing was performed on MinION (Oxford Nanopore Technologies (ONT), Oxford, UK) and Illumina (Illumina Inc, San Diego, US) platforms [ ]. .. Sequencing libraries for MinION were prepared from mechanically fragmented DNA (g-TUBE, Covaris) using the ONT 1D ligation sequencing kit (SQK-LSK108) with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore). .. MinION sequencing was done on a R9.4 SpotON flow cell with a MinION MK1b device.

Article Title: Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same sequence types reveals diversity in prophages and pathogenicity islands.
Article Snippet: Whole-genome sequencing was performed on MinION (Oxford Nanopore Technologies (ONT), Oxford, UK) and Illumina (Illumina Inc, San Diego, US) platforms [12]. .. Sequencing libraries for MinION were prepared from mechanically fragmented DNA (g-TUBE, Covaris) using the ONT 1D ligation sequencing kit (SQK-LSK108) with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore). .. MinION sequencing was done on a R9.4 SpotON flow cell with a MinION MK1b device.

Ligation:

Article Title: Complete Genome Sequence of a Canadian Klebsiella michiganensis Strain, Obtained Using Oxford Nanopore Technologies Sequencing
Article Snippet: Genomic DNA was extracted and purified from overnight cultures grown in TSB at 37°C using the Nanobind CBB Big DNA kit (cells, bacteria, blood) followed by the Short Read Eliminator XS kit (Circulomics, Inc., Baltimore, MD, USA) and was quantified using a Qubit v3.0 fluorometer (Life Technologies, Thermo Fisher Scientific, Inc., Ottawa, Canada). .. Libraries for MinION sequencing were prepared without shearing using the 1D ligation sequencing kit (SQK-LSK108), and DNA was barcoded with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore Technologies, Oxford, UK). .. The final library was run on a FLO-MIN106 (R9.4.1) flow cell for 48 h on the MinION platform (Oxford Nanopore Technologies).

Article Title: Noninvasive prenatal testing for β-thalassemia by targeted nanopore sequencing combined with relative haplotype dosage (RHDO): a feasibility study
Article Snippet: The size distribution of the libraries was analyzed using an Agilent 2100 bioanalyzer and library concentration was quantified by Qubit (Life Technologies). .. Then the sequencing libraries were prepared employing the Oxford ligation sequencing kit SQK-LSK108 (Oxford Nanopore Technologies) and pooled in equal concentration using native barcoding expansion kit EXP-NBD103 (Oxford Nanopore Technologies) according to recommended procedure. .. The library was subjected to 48 h reads generated on the MK1B MinION platform using the R9.4 (FLO-MIN106) flow cell (Oxford Nanopore Technologies).

Article Title: Complete Genome Sequence of a Canadian Klebsiella michiganensis Strain, Obtained Using Oxford Nanopore Technologies Sequencing
Article Snippet: Genomic DNA was extracted and purified from overnight cultures grown in TSB at 37°C using the Nanobind CBB Big DNA kit (cells, bacteria, blood) followed by the Short Read Eliminator XS kit (Circulomics, Inc., Baltimore, MD, USA) and was quantified using a Qubit v3.0 fluorometer (Life Technologies, Thermo Fisher Scientific, Inc., Ottawa, Canada). .. Libraries for MinION sequencing were prepared without shearing using the 1D ligation sequencing kit (SQK-LSK108), and DNA was barcoded with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore Technologies, Oxford, UK). .. The final library was run on a FLO-MIN106 (R9.4.1) flow cell for 48 h on the MinION platform (Oxford Nanopore Technologies).

Article Title: Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same sequence types reveals diversity in prophages and pathogenicity islands
Article Snippet: Whole-genome sequencing was performed on MinION (Oxford Nanopore Technologies (ONT), Oxford, UK) and Illumina (Illumina Inc, San Diego, US) platforms [ ]. .. Sequencing libraries for MinION were prepared from mechanically fragmented DNA (g-TUBE, Covaris) using the ONT 1D ligation sequencing kit (SQK-LSK108) with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore). .. MinION sequencing was done on a R9.4 SpotON flow cell with a MinION MK1b device.

Article Title: Genomic insights into methicillin-resistant Staphylococcus pseudintermedius isolates from dogs and humans of the same sequence types reveals diversity in prophages and pathogenicity islands.
Article Snippet: Whole-genome sequencing was performed on MinION (Oxford Nanopore Technologies (ONT), Oxford, UK) and Illumina (Illumina Inc, San Diego, US) platforms [12]. .. Sequencing libraries for MinION were prepared from mechanically fragmented DNA (g-TUBE, Covaris) using the ONT 1D ligation sequencing kit (SQK-LSK108) with the native barcoding expansion kit (EXP-NBD103) (Oxford Nanopore). .. MinION sequencing was done on a R9.4 SpotON flow cell with a MinION MK1b device.

Concentration Assay:

Article Title: Noninvasive prenatal testing for β-thalassemia by targeted nanopore sequencing combined with relative haplotype dosage (RHDO): a feasibility study
Article Snippet: The size distribution of the libraries was analyzed using an Agilent 2100 bioanalyzer and library concentration was quantified by Qubit (Life Technologies). .. Then the sequencing libraries were prepared employing the Oxford ligation sequencing kit SQK-LSK108 (Oxford Nanopore Technologies) and pooled in equal concentration using native barcoding expansion kit EXP-NBD103 (Oxford Nanopore Technologies) according to recommended procedure. .. The library was subjected to 48 h reads generated on the MK1B MinION platform using the R9.4 (FLO-MIN106) flow cell (Oxford Nanopore Technologies).

Size Selection:

Article Title: Rapid culture-based identification of Shiga toxin-producing Escherichia coli and Shigella spp./Enteroinvasive E. coli using the eazyplex® EHEC complete assay.
Article Snippet: Shiga toxin–producing Escherichia coli (STEC) and Shigella spp./enteroinvasive E. coli (EIEC) are common diarrheagenic bacteria that cause sporadic diseases and outbreaks.. Clinical manifestations vary from mild symptoms to severe complications.. For microbiological diagnosis, culture confirmation of a positive stool screening PCR test is challenging because of timeconsuming methods for isolation of strains, wide variety of STEC pathotypes, and increased emergence of non-classical strains with unusual serotypes.

Generated:

Article Title: Rapid culture-based identification of Shiga toxin-producing Escherichia coli and Shigella spp./Enteroinvasive E. coli using the eazyplex® EHEC complete assay.
Article Snippet: Shiga toxin–producing Escherichia coli (STEC) and Shigella spp./enteroinvasive E. coli (EIEC) are common diarrheagenic bacteria that cause sporadic diseases and outbreaks.. Clinical manifestations vary from mild symptoms to severe complications.. For microbiological diagnosis, culture confirmation of a positive stool screening PCR test is challenging because of timeconsuming methods for isolation of strains, wide variety of STEC pathotypes, and increased emergence of non-classical strains with unusual serotypes.

Nanopore Sequencing:

Article Title: Rapid culture-based identification of Shiga toxin-producing Escherichia coli and Shigella spp./Enteroinvasive E. coli using the eazyplex® EHEC complete assay.
Article Snippet: Shiga toxin–producing Escherichia coli (STEC) and Shigella spp./enteroinvasive E. coli (EIEC) are common diarrheagenic bacteria that cause sporadic diseases and outbreaks.. Clinical manifestations vary from mild symptoms to severe complications.. For microbiological diagnosis, culture confirmation of a positive stool screening PCR test is challenging because of timeconsuming methods for isolation of strains, wide variety of STEC pathotypes, and increased emergence of non-classical strains with unusual serotypes.



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